Graphical interpretation of Boolean operators for protein NMR
We have developed a graphics based algorithm for semi-automated
protein NMR assignments. Using the basic sequential triple resonance
assignment strategy, the method is inspired by the Boolean operators as
it applies "AND"-, "OR"- and "NOT"-like operations on planes pulled out
of the classical three-dimensional spectra to obtain its functionality.
The method's strength lies in the continuous graphical presentation of
the spectra, allowing both a semi-automatic peaklist construction and
sequential assignment. We demonstrate here its general use for the case
of a folded protein with a well-dispersed spectrum, but equally for a
natively unfolded protein where spectral resolution is minimal.